Factors affecting cold hardiness in the small striped flea beetle, Phyllotreta undulata

摘要

The striped flea beetle, Phyllotreta undulata Kutschera (Coleoptera: Chrysomelidae), is a pest of cruciferous crops. It overwinters as an adult. During winter in northern European countries, such as Estonia, it is subject to sometimes severe temperatures that may fluctuate daily, over the season, and between seasons. The objective of this study was to investigate factors that affect its cold hardiness. In a series of five experiments, the effects of food plant, starvation, and acclimatization on the beetles' ability to supercool and survive exposure to sub-zero temperatures was investigated. The supercooling points (SCP) of overwintered beetles field-collected from white mustard and Indian mustard differed from those caught from white cabbage and oilseed rape, but these differences disappeared after a 4-day period of starvation at room temperature, indicating that gut content probably influences the potential to supercool. The duration and temperature of acclimation affected SCP in overwintered beetles. The decrease in SCP was more rapid at 22 pC than at 0 pC, probably because of faster dehydration and gut evacuation at the higher temperature. Acclimation at 0 pC for a week increased the ability of overwintered beetles to survive sub-zero temperatures, lowering both SCP and lower lethal temperature (LLT). Some pre-freeze mortality occurred; SCP and LLT were correlated but the latter was a constant 3 pC higher than the former. The SCP of field-collected pre-winter beetles decreased gradually during the autumn. It also decreased when field-collected pre-winter beetles were acclimated at 0 pC in the laboratory, attaining its lowest level after 18 days. Phyllotreta undulata is well-adapted to unstable and sometimes severe winter conditions; its high potential to supercool enhances its cold hardiness and ability to survive short periods at sub-zero temperatures although it cannot survive freezing of its body fluids.