Changes in chlorophyll and nitrogen content in blackgram mutant lines induced by different mutagenic treatments towards root-knot nematode infection.

摘要

A replicated pot culture experiment was designed to investigate the changes of nitrogen and chlorophyll content in superior mutant lines (M₅) developed from three morphologically different blackgram varieties (Pant U-30, PDU-I and Sarala) towards M. incognita infection. Nematode inoculation reduced chlorophyll-a, chlorophyll-b, total chlorophyll, nitrogen content in shoot of blackgram by 18.99, 22.5, 21.24 and 11.41% respectively, but inoculated plant roots recorded 1.761% nitrogen which was 10.06% increase over the uninoculated plants. Sarala variety recorded higher quantities of chlorophyll-a (1.794 mg/g), chlorophyll-b (1.350 mg/g) total chlorophyll (3.152 mg/g), nitrogen in shoot (3.053%) and nitrogen in root (1.815%) as compared to other two parental varieties on these biochemical characters. EMS (0.3%) treatment induced three characters such as chlorophyll-a, chlorophyll-b and total chlorophyll by 20.5, 88.4 and 46.4% increase over control respectively. Gamma ray (30 kR) induced higher percentage of nitrogen in shoot (48.4) and root (22.5). NG (0.03%) was superior to gamma ray treatment for chlorophyll-a, chlorophyll-b and total chlorophyll.