Role of Auxins, Sucrose and Other Matrices on in vitro Rooting of Shoots of Chlorophytum borivilianum Sant. et Fernand

摘要

Chlorophytum borivilianum is an endangered and important plant species of Indian system of medicine, belongs to the family Liliaceae. A cost effective method for in vitro rooting in the shoots of this species was developed. Rapid multiplication was obtained on MS medium through inflorescence nodal segments with higher concentration of Nu6-benzyl adenine. But further shoots multiplication with healthy plantlets was achieved on MS medium fortified with less concentration of BA in combination with adenine sulfate. The regenerated shoots were transferred to MS medium with different concentrations of auxins and sucrose. The rooting was carried out on MS medium with different matrices like glass wool, Luffa sponge and moss instead of agar. However, higher number of roots with good thickness was obtained on MS ^% liquid medium with 0.5 mg/l IBA with 2% sucrose and 3% sucrose with glass wool which indicates the suitability over others. The rooted shoots were acclimatized and transferred to the field with 83% success.