Isolation of the uma-4 gene encoding the 26 kDa subunit of the Neurospora crassa vacuolar ATPase

摘要

We have isolated the vma-4 gene, which encodes a 25 746 Dalton subunit of the vacuolar ATPase, from Neurospora crassa. The gene contains two introns and was mapped to the left arm of linkage group 1. Comparison of the predicted amino acid sequence with homologous proteins from Saccharomyces cerevisiae, Manduca sexta, and Bos taurus showed only 25% sequence identity. However, computer-assisted predictions of secondary structures gave similar results for all four proteins. Analysis of the sequence and the available biochemical data indicated that the vma-4 gene product may play the same structural role in the vacuolar ATPase as does the γ-subunit in F-type ATPases.