A cost-effective and reliable method to predict mechanical stress in single-use and standard pumps

摘要

The suitability of oil-water emulsions to predict shear forces in stirred bioreactors under cost-effective and time-saving conditions has been demonstrated several times, but no application to pumps has been described so far. In this report, the drop sizes in a model oil-water system were determined for the Levitronix PuraLev (R) multiuse (MU) series (PuraLev (R) 200MU and PuraLev (R) 600MU), a peristaltic pump (Masterflex (R) I/P Easy Load), and 4-piston diaphragm pump (Quattroflow 1200-SU, where SU is single-use) using inline endoscopy. It was determined that the Sauter mean diameter could be used as a comparison criterion to estimate mechanical stress in pumps. The investigation showed that PuraLev (R) MU pumps are characterized by up to 59% larger Sauter mean diameters than their counterparts at comparable operational conditions. This indicates lower hydrodynamic stress in the PuraLev (R) MU pumps. Using computational fluid dynamics, a well-streamlined fluid flow and low turbulent energy dissipation rates were found in the PuraLev (R) MU pumps, which correlated well with experimental results. A calculation model was used to predict the Sauter mean diameter by combining both experimental and computational fluid dynamics data. Good agreement with deviations below 13% was determined between model predictions and experimental data