Voltage gated potassium channels in the larval CNS neurons of coconut black headed caterpillar, Opisina arenosella Wlk.

摘要

A primary dissociated cell culture of Opisina arenosella Wlk. larval Central Nervous System (CNS) neurons was established. The acutely dissociated larval CNS neurons could be divided into two categories based on their morphology and size. Type I consisted of rounded cells and type II consisted of cells with processes. Nearly 60 per cent of the cells were alive after 7 days of plating. Whole cell currents were recorded from Type II cells (5-12 )mum) of 12-48 h using the patch clamp setup. The outwardpotassium currents were obtained from the neuronal cells. The absence of potassium currents from the cells when exposed to potassium channel blockers (cocktail of 4-aminopyridine (1 mM), quinidine (0.1 mM) and tetraethylammonium (TEA, 20 mM) in extra cellular bath) confirmed the potassium currents. Three types of potassium currents (non inactivating, slow inactivating, and fast inactivating currents) were observed in whole cell recording of Type II cells.