Computational Multiple Steady States of the Penicillin G Acylase-Catalyzed Hydrolysis in an Isothermal Continuous Flow Stirred Tank Reactor

摘要

A family of an enzymatically catalyzed reaction network was studied, which involves the hydrolysis of penicillin G by penicillin G acylase in an isothermal continuous flow stirred tank reactor (CFSTR). This system consisted of 10 coupled non-linear equations and was found to be capable of exhibiting computational multiple steady states. A set of kinetic parameters determined from the existing experimental data were used to compute a set of rate constants and two corresponding steady states. This suggested that multiple steady states may occur in the system studied. The phenomena of bistability, hysteresis and bifurcation were discussed. Moreover, the capacity of steady state multiplicity was extended to its family of reaction networks.