Ethanol- and acetonitrile-induced inhibition of water diffusional permeability across bovine red blood cell membrane

摘要

The effect of 0–3% (v/v) ethanol and acetonitrile on water diffusional permeability of bovine and chicken red blood cells (RBCs) was studied using a pulse 1H-T2 NMR technique. Transmembrane water diffusional exchange times, τexch, of 9.2 ± 0.46 ms and 18.3 ± 1.0 ms were determined for bovine and chicken erythrocytes at 27.5° C, respectively. Arrhenius activation energies Ea of water diffusion were 20.4 and 35.8 kJ mol?1. Ethanol, and acetonitrile being 2-fold more effective, markedly increased both τexch and Ea in bovine RBC as compared to the well-known mercurial inhibitor of water channels, p-chloromercuribenzene sulfonate. Chicken RBCs that have no protein water channels, were found to be completely insensitive for either agent. It was suggested that ethanol and acetonitrile partitioning into the lipid phase of bovine RBC membrane affects the permeability of CHIP28 water channel but not the lipid confined water diffusion. The results suggest that the inhibition of transmembrane movement of water via CHIP28 channels might be involved in the anti-hemolytic action of anaesthetics such as ethanol.