Application of Mid-Infrared Molecular Spectroscopy for Assessment of Biochemical Parameters of Blood Serum

摘要

The method of mid-infrared molecular spectroscopy allows precise measuring of the concentrations of a large number of biological molecules in a minimal sample volume. Method of projections on latent structures was used for plotting the calibration models. On the basis on mid-infrared spectral data we obtained calibration models for calculation of serum content of various substances: albumin, cholesterol, glucose, total protein, urea, 70 Da heat shock protein, and malonic dialdehyde.The search for precise, reproducible, and simple methods of simultaneous analysis of concentration of several substances in a small volume of biological material is a priority problem of biological analysis.