Study on interaction of hemin with pyronarine and analytical application in salt bridge supported bilayer lipid membrane system

摘要

Pyronarine (PY) is a novel antimalarial drug. The interaction of hemin with PY was investigated in salt bridge supported bilayer lipid membrane (Sb-HLM) system. Sb-BLM was modified with anionic site laurie acid anions (LA). Then, hemin was also adsorbed and incorporated in Sb-BLM by electrostatic force between hemin and LA. Hemin showed well cyclic voltammetry behavior. The cathodic peak current I{sub}(pc) of hemin decreased in the presence of PY, which illustrated the interaction between hemin and PY. The I{sub}(pc) decreases proportional to the increase of PY concentration from 2.75 × 10{sup}(-9) to 3.02× 10{sup}(-5) mol{sup}(-1), which is the basis for the determination of PY. The interacting mechanism was studied and the optimum conditions for assay of PY were also investigated.