Reconstitution of the B800 bacteriochlorophylls in the peripheral light harvesting complex B800-850 of Rhodobacter sphaeroides 2.4.1 with BChl a and modified (bacterio-)chlorophylls

摘要

A method is described for reversibly removing bacteriochlorophyll from the B800-site of the B850-850 antenna complex from Rhodobacter sphaeroides. This method uses the oligosaccharidic detergent Triton BG-10, Together with an incubation at pH 5.0. Reconstitution at the B800-site has been successfully achieved for a range of modified bacteriochlorophylls.