Modulation of Ca~(2+) Influx into a Single Rice Cell with an Electric Signal

摘要

A pulsing electric signal was applied to a target rice cell with a pair of Pt plate electrodes to modulate the Ca~(2+) influx into the cells. The change of intracellular concentration of Ca~(2+) was measured continuously. The cross membrane potential (|V_CMP|) generated at the cell membrane was analyzed on the basis of the measured data of potential gradient at the very position of the cell. When |V_CMP| was no higher than 1.0 mV, no appreciable influx of Ca~(2+) was observed. In contrast, when |V_CMP| was higher than 1.9 mV, a marked increase in the Ca~(2+) influx occurred. This influx was inhibited by verapamil, a Ca~(2+) channel blocker, which suggested the involvement of Ca~(2+) channel. Therefore, the Ca~(2+) influx into a rice cell can be modulated with a pulsing electric signal via potential sensitive Ca~(2+) channels.