Amperometric monitoring of redox activity in living yeast cells: comparison of menadione and menadione sodium bisulfite as electron transfer mediators

摘要

An amperometric method was applied for real-time monitoring of intracellular redox enzyme activity. Baker's yeast (Saccharomyces cerevisiae) cells were immobilized on platinum microband electrodes and mediated anodic currents were measured. The currents were observed in the absence and in the presence of glucose as a source of reducing equivalents, NADH and NADPH. 2-Methyl-1,4-naphthoquinone (menadione, vitamin K_3) and water soluble 2-methyl-1,4-naphthoquinone sodium bisulfite (menadione sodium bisulfite MSB) were compared as artificial electron acceptors for their ability to transduce internal cellular redox activity into electrode current. It was found that hydrophobic menadione was superior to its water-soluble bisulfite derivative for probing whole intact cells.