A new self-assembled modified electrode for competitive immunoassay

摘要

Alternating films constructed upon successive deposition of redox polymer Os (bpy)_2ClPyCH_2NHpoly (allylamine) and antibiotin IgG were developed for amperometric immunosensor design. Cyclic voltammetric measurements were used to verify charge transport between redox sites and the redox surface concentration was estimated upon voltammetric peak integration. Biotin-antibiotin complex formation was evaluated using horseradish peroxidase as an enzyme label. Redox mediation between the modified electrode surface and the redox site in the enzyme was verified after substrate addition. Multilayer modified gold electrodes with biologically active antibiotin IgG molecules were employed for the development of a competitive immunoassay with electrochemical label detection.