Morphofunctional Evaluation of the Effect of Collagen-1-Based Dressing on Skin Regeneration after Burn Trauma in Mice of Two Genetic Strains

摘要

Morphofunctional evaluation of the effect of biological dressing with collagen-1 on healing of 3 A degree burn wound in outbred and mutant Hr~hr/Hr~hr (hairless) mice was carried but by the histological method and optic radioautography. A pronounced stimulatory effect of the dressing on skin regeneration in mice was demonstrated. According to radioautography data, early dressing of the bum wounds in Hr~hr/Hr~hr mice led to active proliferation of epithelial cells in dermal cyst and vascular endotheliocytes. The possible mechanisms of the stimulatory effect of collagen-based dressing on wound healing are discussed.The physiological reaction of the skin epidermis is realized at the expense of its basal layer stem cells. If the basal layer is damaged, stem cells from the skin appendages (hair follicle (HF), sebaceous and sweat glands) are involved in regeneration of the epidermis [12]. Mutation in the Hairless (Hr) gene in mice causes destruction of HF normally formed during em-bryogenesis, which leads to complete alopecia in these animals [16]. This mouse strain homozygotic by Hr gene mutant allele is used as a model for studies of the mechanisms of HF functioning and for the development of methods for the treatment of hair growth diseases. Phenotypical manifestation of Hr gene mutation in mice is similar to human hereditary dermal disease, papular atrichia [17]. Therefore, study of the functional characteristics of Hr gene improves our understanding of human skin physiology.