Electrochemical detection of MS2 age using a bead-based immunoassay and a NanoIDA

摘要

A microbead based sandwich immunoassay for MS2 bacteriophage was developed using an interdigitated array (IDA) electrode with nanoscale dimensions (220 urn electrode width, 620 nm gap). The IDA was fabricated using an electron beam lithographic lift-off technique. After an antibody-assisted capture of MS2 using paramagnetic microbeads, a beta-galactosidase labeled secondary antibody was used to convert p-aminophenyl-beta-D-galactopyranoside (PAPG) into the redox active p-aminophenol (PAP). Amperometric detection of PAP with IDA electrodes at +300 and -200 mV vs. a Ag/AgCl reference electrode was used to measure the result, detecting MS2 concentrations as low as 10 ng/mL.