Development of a Catalase-Based Amperometric Biosensor for the Determination of Increased Catalase Content in Milk Samples

摘要

The aim of our work was to develop a selective amperometric biosensor for infected milk detection. The catalase enzyme (EC 1.11.1.6) was immobilized onto the surface of a thin-layer enzyme cell. The measurements were carried out in stopped-flow injection analysis system (0.8mLmin ~(-1) flow rate, +590mV polarization potential) in organic phase (6% sodium acetate buffer 200mM pH6.0, ferrocene conductive salt (7.5mgL ~(-1)) dissolved in acetonitrile. A certain amount of hydrogen peroxide was degraded by the catalase content of the milk. The degradation of the hydrogen peroxide is proportional to the infection of the milk samples.