Interaction Between DNA and Some Salicylic Acid Derivatives and Characterization of Their DNA Targets

摘要

A nanofiber polypyrrole (PPy) film was electrochemically deposited on a Pt electrode and used for immobilization of single-stranded DNA (ssDNA) and investigation of hybridization events. Then, the interaction of DNA with four salicylic acid (SA) derivatives was studied with electrochemical methods. The oxidation peak of guanine was decreased by increasing the concentrations of salicylic acid derivatives. The binding constants of these compounds with four different sequences of DNA including different percentages of guanine-cytosine and adenine-thymine bases were calculated and it was clarified that sequences with higher percentage of adenine-thymine bases have a higher binding constant in their interaction with SA derivatives.