The New Application of Boron Doped Diamond Electrode Modified with Nafion and Lead Films for Simultaneous Voltammetric Determination of Dopamine and Paracetamol

摘要

A new voltammetric procedure for the simultaneous determination of dopamine (DA) and paracetamol (PA) using boron doped diamond electrode modified with Nafion and lead films (PbF/Nafion/BDDE) was investigated. The use of this electrode resolved the overlapped voltammetric waves of DA and PA into well- defined peaks with peak to peak separation of about 320 mV. Under the optimized experimental conditions in differential pulse voltammetric technique, DA and PA gave a linear response over the ranges 2.0 x 10(-7)- 1.0 x 10(-4) mol L-1*(R-2 = 0.9996) and 5.0 x 10(-) 7-1.0 x 10(-3) mol L-1 (R-2 = 0.9979), respectively. The detection limits were found to be 5.4 x 10(-)8 mol L-1 for DA and 1.4 x 10(-7) mol L-1 for PA. They are lower, comparable or in some cases a little bit higher than those obtained using other electrochemical sensors. However, the proposed procedure of the sensor preparation is much simpler than procedures described in the literature with a lower detection limit. The proposed procedure was successfully applied to the determination of PA in some commercial pharmaceuticals as well as to the simultaneous determination of DA and PA in human urine, whole blood and serum samples directly without any separation steps.