Filtration method for studies of the kinetics of hypo-osmotic pore closure in erythrocyte.

摘要

Filterability of erythrocytes through small (3 mu) pores decreases with decreasing osmolarity of suspension medium because of hypo-osmotic swelling of cells. After appearance of lytic pores, erythrocyte filterability increases for some time, while after recovery of membrane integrity it decreases again. We suggest filtration method for studies of the kinetics of hypo-osmotic lytic pores closure. The dynamics of changes in erythrocyte filterability was studied in 2 patients with paroxysmal nocturnal hemoglobinuria and 6 donors (Ht 0.01%, Na phosphate buffer 5 mM, pH 7.4, 35 mOsm, 24 degrees C). The method can be used for studies of erythrocyte membrane characteristics in various diseases and for evaluation of the membranotropic effects of drugs, infusion media, hemolysins, ethanol, etc.