The use of immunohistochemical method for detection of brain microglia in paraffin sections.

摘要

We developed a well reproducible method for detection of brain microglia on paraffin sections on the basis of modern immunohistochemical methods. Polyclonal antibodies to Iba1 antigen obtained after immunization with a synthetic peptide corresponding to 81-93 amino acid fragment of Iba1 antigen selectively interact with microglia cells on paraffin sections of rat brain. Detailed protocol of preparation treatment was drawn up ensuring complete detection of the microglia expressing Iba1 protein.