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首页> 外文期刊>Bulletin of experimental biology and medicine >Evaluation of Serum Antioxidant Activity in Patients with Liver Pathology by the Chemiluminescent Method
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Evaluation of Serum Antioxidant Activity in Patients with Liver Pathology by the Chemiluminescent Method

机译:化学发光法评价肝病患者血清抗氧化活性

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摘要

Total antioxidant activity of the serum in patients with liver pathology was assessed by two chemiluminescent methods based on different models of free radical oxidation: Hb-H2O2-luminol and ABAP-luminol. Comparative analysis showed a significant, but not high correlation of the results (r=0.798), which can be explained by different mechanisms of induction of free radicals and effects of various serum components (proteins and bilirubin) on the initiation process. The influence of aphysiological concentration of analyzed values manifested in the Hb-H2O2 model. Disagreement between the results of measurements was more pronounced in patients with abnormally high serum bilirubin content. The results suggested that ABAP-luminol chemiluminescent model is more preferable for evaluation of antioxidant activity in clinical practice.
机译:根据两种不同的自由基氧化模型,通过两种化学发光方法评估了肝病患者血清中的总抗氧化活性:Hb-H2O2-鲁米诺和ABAP-鲁米诺。对比分析显示结果具有显着但不高度的相关性(r = 0.798),这可以用自由基诱导的不同机制以及各种血清成分(蛋白质和胆红素)对引发过程的影响来解释。生理浓度分析值的影响体现在Hb-H2O2模型中。在血清胆红素含量异常高的患者中,测量结果之间的分歧更为明显。结果表明,ABAP-鲁米诺化学发光模型在临床实践中更优选用于评估抗氧化剂活性。

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