Antitumor and immunomodulatory effects of culinary-medicinal Shiitake mushroom Lentinus edodes (Berk.) Singer: Analysis of NK activity, lymphoproliferative response, and antibody production

摘要

We evaluated the efficiency of 12 aqueous extracts obtained at three different temperatures from four strains of culinary-medicinal Shiitake mushroom Lentinus edodes in protecting mice against the development of Ehrlich carcinoma. We observed first that the strains Le 96/17 and Le 95/01 of L. edodes were more efficient than JAB/k and Le 96/22 in increasing the survival time of tumor-bearing mice and that the temperature of 60degreeC for extraction was more efficient than 22degreeC or 100degreeC for the first two strains. Groups of animals were then sacrificed following the treatments with the extract from Le 96/17 or Le 95/01 obtained at 60degreeC and evaluated on the NK activity, the lymphoproliferative responsiveness of spleen cells to concanavalin A, and the specific antibody production. NK activity and proliferative response were not affected by the treatments, whereas the antitumor antibody response was enhanced by Le 95/01. Further analysis demonstrated that the extracts in vitro inhibited the NK activity and the proliferative responsiveness to concanavalin A.