A method for making preparations from nondecalcified articular cartilage with sublying subchondral bone for multipurpose studies

摘要

A method for making preparations from nondecalcified articular cartilage and subchondral bone for multipurpose studies is developed. Semithin and ultrathin sections of nondecalcified articular cartilage and subchondral bone prepared by this method are used for light and transmission electron microscopy. The method can also be used for studies of the qualitative and quantitative characteristics of the articular cartilage, scanning electron microscopy, and microanalysis of chemical elements in the articular cartilage and subchondral bone.