首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >A QUANTITATIVE RELATIONSHIP BETWEEN CAPACITY FACTOR AND SELECTOR CONCENTRATION IN CAPILLARY ELECTROPHORESIS AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY - EVIDENCE FROM THE ENANTIOSELECTIVE RESOLUTION OF BENZOIN USING HUMAN SERUM ALBUMIN AS A CHIRAL SE
【24h】

A QUANTITATIVE RELATIONSHIP BETWEEN CAPACITY FACTOR AND SELECTOR CONCENTRATION IN CAPILLARY ELECTROPHORESIS AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY - EVIDENCE FROM THE ENANTIOSELECTIVE RESOLUTION OF BENZOIN USING HUMAN SERUM ALBUMIN AS A CHIRAL SE

机译:毛细管电泳与高效液相色谱法中容量因子与选择剂浓度之间的定量关系-以人血清白蛋白为手性化合物对苯甲醛对映拆分的证据。

获取原文
获取原文并翻译 | 示例
获取外文期刊封面目录资料

摘要

Many selectors are used both in pressure-driven liquid chromatography (LC) and in electrokinetic chromatography (EKC), particularly chiral species such as cyclodextrins and proteins. It should be possible to readily apply information gleaned using one technique to the other, since in both techniques the underlying molecular interactions which lead to separations are expected to be the same. Superficially this may be the case, but an exact transfer of operating conditions, i.e., background electrolyte (BGE) composition/mobile phase composition, assuming that these meet certain minimum requirements for each technique, is not often possible. To investigate the reason for this we have measured retention (k') of a neutral solute (racemic benzoin) in HPLC and EKC using an identical range of BGE/mobile phase conditions in both techniques. The selector used was human serum albumin. The k' measurements obtained for each benzoin enantiomer were consistently higher in HPLC than in EKC. This can be explained very simply if one considers that retention in both systems is related to the selector concentration [S], by the expression k' = K[S], where K is the affinity constant. In EKC, [S] is simply the concentration of free selector in the BGE, while in LC, [S] = m(p)/V-o, where m(p) is the number of moles of accessible selector, and V-o is the column void volume. In LC, [S] is generally considerably higher than in EKC, leading to larger values of k'. [References: 23]
机译:在压力驱动液相色谱(LC)和电动色谱(EKC)中都使用了许多选择器,特别是手性物质,例如环糊精和蛋白质。应该容易地将使用一种技术收集的信息应用于另一种技术,因为在这两种技术中,导致分离的潜在分子相互作用被认为是相同的。表面上可能是这种情况,但是通常不可能精确地转移操作条件,即背景电解质(BGE)组成/流动相组成,前提是这些条件满足每种技术的某些最低要求。为了调查其原因,我们在两种技术中使用相同范围的BGE /流动相条件,测量了中性溶质(外消旋安息香)在HPLC和EKC中的保留(k')。使用的选择剂是人血清白蛋白。 HPLC中每种苯偶姻对映体的k'测量值始终高于EKC。如果人们认为两个系统中的保留率与选择剂浓度[S]有关,则可以用表达式k'= K [S]来非常简单地解释,其中K是亲和常数。在EKC中,[S]只是BGE中自由选择剂的浓度,而在LC中,[S] = m(p)/ Vo,其中m(p)是可接近选择器的摩尔数,Vo是色谱柱空隙体积。在LC中,[S]通常远高于EKC,导致k'更大。 [参考:23]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号