Effects of Deep Cooling and Re-Warming on Ionotropic Glutamatergic Receptors In Vitro

摘要

We studied the effects of cooling to -10A degrees C and re-warming to 37A degrees C on slices of rat olfactory cortex. The amplitudes of action potential in the lateral olfactory tract and excitatory postsynaptic potential activated by AMPA recovered during slow cooling/re-warming (0.1A degrees C/min), while during rapid cooling/re-warming (9A degrees C/min), they surpassed the control values. NMDA receptor-dependent mechanism was blocked in both cooling/re-warming modes. Swelling of the brain slices was observed during re-warming, especially during rapid cooling/re-warming. Nerve fibers of the lateral olfactory tract and AMPA-related processes survived deep cooling/re-warming, while NMDA-related processes were irreversibly blocked.