Chemical characterization of milk oligosaccharides of the brown bear, Ursus arctos yesoensis

摘要

Two tri-, two hexa- and two deca-saccharides were isolated from bear milk by chloroform/methanol extraction and gel filtration. The oligosaccharides were characterized, mainly by 1H-NMR, as follows: Gal α 1-3Gal β 1-4Glc (α3'-galactosyllactose) Fcuα 1-2Gal β 1-4Glc (2'-fucosyllactose) Galα 1-3Gal β 1-4[Fuc α1-3]GlcNAcβ1-3Galβ 1-4Glc (monofucosylhexasaccharide) Fucα 1-2Gal β 1-4[Fuc α1-3]GlcNAcβ1-3Galβ 1-4Glc (difucosylhexasaccharide) Galα 1-3Gal β 1-4[Fuc α1-3]GlcNAcβ1-6Galβ 1-4Glc (difucosyldecasaccharide) Galα 1-3Gal β 1-4[Fuc α1-3]GlcNAcβ1-3Galβ 1-4Glc (difucosyldecasaccharide) Fucα 1-2Gal β 1-4[Fuc α1-3]GlcNAcβ1-6Galβ 1-4Glc (tetrafucosyldecasaccharide) Fucα 1-2Gal β 1-4[Fuc α1-3]GlcNAcβ1-3Galβ 1-4Glc (tetrafucosyldecasaccharide) Both trisaccharides were present in bear milk at a higher concentration than lactose.