Effect of endosulfan on the enzymes of polyol pathway in rat Sertoli-germ cell coculture.

摘要

The effect of endosulfan (at 0, 2.0, 20.0, 40.0 and 80.0μM) on the activities of sorbitol dehydrogenase [L-iditol dehydrogenase] and aldose reductase [aldehyde reductase], measured as nmol NADPH oxidized min-1 -mg protein, was investigated using rat Sertoli-germ cell coculture. The results revealed an increase in the activity of aldose reductase in Sertoli-germ cell coculture exposed to 20-80μM endosulfan when compared with the control and the 2μM endosulfan group both after 24 and 48 h following treatment. The increase in aldose reductase activity was in a dose-dependent manner. Duration dependency was observed only in the higher dosed groups (40-80μM endosulfan). A dose-dependent increase in the activity of sorbitol dehydrogenase was recorded from 20 to 80μM exposed group after 24 h following treatment. However, the sorbitol dehydrogenase activity recorded from culture exposed to 2μM endosulfan was similar to that of the control. The trend of the activity was similar when the exposure time was further increased to 48 h -- a dose-dependent increase in sorbitol dehydrogenase activity from 20 to 80μM endosulfan. No duration-dependent changes was observed in the 48 h treatment. This study suggests that endosulfan disrupts the polyol pathwayby affecting specific enzymes, which may be one of the reasons leading to cytotoxicity in vitro.