Cleanup Method Using Disposable Tandem Cartridge System for the Determination of Dioxins in Human Milk by Enzyme-Linked Immunosorbent Assay

摘要

For dioxin analysis, the establishment of the appropriate analytical method and the quality control procedure are indispensable for reliable measurements. On the other hand, a method that is simple, rapid, inexpensive, and highly sensitive for the screening of dioxins has been highly demanded by the public and the government. One of the methods that may satisfy these requirements is an enzyme-linked immunosorbent assay (ELISA), and there have been some reports on the measurement of dioxins using ELISA. We have already reported the development of a dioxin toxicity evaluation method for human milk by ELISA (Sugawara et al. 1998; Ishizuka et al. 2001; Sugawara et al. 2002; Saito et al. 2003a). However, the dioxin analysis by ELISA usually requires a sufficient cleanup similar to the GC/MS method due to the extremely low content of the dioxins, though ELISA is generally said to have the ability of high specificity.