Production arid application of compatible solute giycine betaine from halophilic bacteria Actinopolyspora halophila

摘要

Cell extracts of Actinopolyspora halophila producing a compatible solute giycine betaine was detected qualitatively by HPTLC and extracted by the process of "bacterial milking". It was found that milking along with purification by ammonium reineckateprecipitation increases the releases of giycine betaine out of the cell by 4.4-folds. The cryoprotectant activity of giycine betaine when used in combination with Sucrose/BSA, for lyophilization of sensitive cultures, allows one to two log cycle increasein viable counts of test cultures as compared to the mixture of only Sucrose/BSA and, hence, is a more effective cryoprotectant for lyophilization of Escherichia coll, Staphylococcus aureus and Vibrio cholerae.