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Isolation and characterization of polymorphic microsatellite markers for the masked palm civet (Paguma larvata).

机译:蒙面灵猫(Paguma larvata)的多态微卫星标记的分离和鉴定。

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摘要

The masked palm civet (Paguma larvata) has been suspected to be the host of a SARS-like CoV virus that causes severe acute respiratory syndrome in humans. In China, the palm civet lives wild and is farmed, but even though the species is a potential carrier of the virus, its geographic distribution and genetic diversity have never been studied. We report the isolation and characterization of six polymorphic microsatellite markers for P. larvata. To characterize each locus, two farmed masked palm civet populations from Shanxi and Guangxi provinces in China were genotyped. The number of alleles per locus ranged from 3 to 15, and the observed heterozygosity for these populations was 47.1 and 68.7%, respectively.
机译:有人怀疑带面具的棕榈灵猫(Paguma larvata)是SARS样冠状病毒的宿主,该病毒会导致人类严重的急性呼吸综合症。在中国,棕榈果子狸生活在野生环境中并被种植,但是即使该物种是该病毒的潜在携带者,也从未研究过其地理分布和遗传多样性。我们报告分离和表征六个多态性微卫星标记的幼虫。为了表征每个基因座,对来自中国山西和广西两省的两种养殖的蒙面棕榈果子狸种群进行了基因分型。每个基因座的等位基因数目范围为3至15,观察到的这些人群的杂合度分别为47.1和68.7%。

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