Analysis of saline activator solution effects on sperm quality indices of Barbus sharpeyi by image J software.

摘要

Regarding low fertilization rate of Barbus sharpeyi, one of the main Barbus species in south of Iran, saline activator solutions (SAS 1 and 2) were used for increasing of sperm activation and its sperm quality indices were assessed by using Image J software as a fish sperm adapted tools. SAS 1 (45 mM NaCl, 5 mM KCl, 30 mM Tris, 188+or-2.3 mosmol/kg) and SAS 2 (50 mM NaCl, 30 mM KCl, 30 mM Tris, 189+or-1.7 mosmol.kg) were added to a mixture of 12 male semen comparing with distilled and tap water and their effects on VCL, VSL, ALH, Linearity, %motility, spermatozoa path, % A, B and C spermatozoa type, fertilization, hatching and deformity rate were analyzed in triplicate. Results showed that SAS2 had the highest VCL (21.37+or-0.36 micro m/s-1), VSL (11.6+or-0.3 micro m/s-1) and motility percentage (85.75+or-1.56%) among treatments. The highest A type spermatozoa percentage was recorded for SAS1 (47.16+or-3.15%) and SAS2 (43.89+or-4.54%). The lowest C type spermatozoa percentage was recorded using SAS2 (14.24+or-1.56%). Also, the highest fertilization rate was achieved from SAS2 (79.5+or-0.37%) and SAS1 (77.74+or-4%) respectively. Furthermore the lowest larval deformity rate (13.77+or-1.03%) was shown due to the use of SAS2. Final conclusion confirmed that SAS1 and SAS2 have positive and significant effects (P<0.05) on extending the time length of sperms movement, VCL and VSL of B. sharpeyi which consequently resulted in better efficiency of propagation of this species in south of Iran.