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首页> 外文期刊>Irish Journal of Agricultural and Food Research >A note on the use of FTA (TM) technology for storage of blood samples for DNA analysis and removal of PCR inhibitors
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A note on the use of FTA (TM) technology for storage of blood samples for DNA analysis and removal of PCR inhibitors

机译:关于使用FTA(TM)技术存储血液样本以进行DNA分析和去除PCR抑制剂的说明

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摘要

FTA (TM) technology is widely used across many molecular disciplines for sample capture, storage and analysis. The use of this technology for the long-term storage of blood samples for DNA analysis was examined as well as its potential to remove inhibitors from DNA samples previously extracted from blood with PCR inhibitors remaining. It was found that blood spots stored on FTA (TM) cards for 8 years at room temperature gave successful PCR products and that FTA (TM) cards are a useful tool for removing substances in samples which interfere with or inhibit, the PCR reaction.
机译:FTA(TM)技术已在许多分子学科中广泛用于样品捕获,存储和分析。检验了该技术在长期保存血液样本中进行DNA分析的用途以及其从先前从血液中提取的DNA样本中去除抑制剂的潜力,并保留了PCR抑制剂。发现在室温下在FTA(TM)卡上存储8年的血斑可成功获得PCR产物,而FTA(TM)卡是去除样品中干扰或抑制PCR反应的物质的有用工具。

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