Development of Technique for Obtaining Transgenie Sorghum Plants by Agrobacterium-Mediated Transformation In Planta

摘要

Development of simple and efficient methods for obtaining genetically engineered plants is one of the main goals of plant biotechnology. During the past years a few reports on obtaining transgenic sorghum plants have been published (Casas et al. 1993,1997). These experiments used the "microbombardment" technique, which needs expensive equipment and laborious steps for obtaining embryogeniq callus, and for selection and regeneration of transformed plants. Moreover reported frequencies of transgenic plants were very low. Another method of genetic transformation based on cocultivation of A.tumefaciens cells with callus or explants tissues is unsuitable for sorghum because of frequent necrosis of sorghum cells after cocultivation (Carvalho 1998; our unpublished results). Development of a simple and reliable method for obtaining transgenic sorghum plants was the main purpose of our investigations.