Long-Term Regeneration in Callus Culture of Paisa (Echinochloa frumentacea Link.)

摘要

Paisa (Echinochloa frumentacea Link.) is an annual fodder cultivated in central Russia's Evropian region (near the city of Orel). The yield of paisa has been reported as high as 60 t ha~(-1) and seed production of 21 ha~(-1). Vegetative growth periodranges from 110 to 125 days, which is too long to obtain mature seed. No significant difference for this trait was found in our plant breeding material. Therefore, the use of biotechnological tools is being exploited to assist in the improvement of the crop. Regeneration in the genus Echinochloa has been based on deriving shoots in callus cultures from mature E. oryzicola seed (Maeda and Sugiura 1976), regenerated plants in cultures of E. oryzicola leaves (Takahashi et al. 1984), from segments of youngE. crusgalli and E. colonum (L). Link, inflorescences (Wang and Yan 1984; Tyagi et al. 1985), from the mesocotyle of E. crusgalli var. oryzicola and E. muricata (Cobb et al. 1985), and in cultures of immature E. glabrescens Munro ex Hook F. inflorescences and leaves (Wang and Zapata 1987). This research was undertaken to evaluate the long-term regeneration of paisa through callus culture.