Characters of plants regenerated from protoplasts of photoperiod-sensitive genie male sterile rice

摘要

Identifying photoperiod-sensitive genie male sterility (PGMS) in rice (Shi 1981) makes it possible to produce hybrid rice seed through the two-line system. We studied the response of PGMS protoplast clones to photoperiod and temperature. variation inploidy, and agronomic traits. Promising PGMS line Zhenongdal 1 s (X126-11) has already been developed through protoplast culture. A cell suspension with small globular calli was established from the scutellum of . mature embryos of PGMS cultivarN5047s. A high density (1.5 × 10~6 ml~(-1)) of protoplasts was obtained, and green regenerates (plantlets) were produced following Xue and Earle's (1995) method.