首页> 外文期刊>International Journal of Pest Management >A 24-h potato-based toxicity test for evaluating Bacillus thuringiensis var. israelensis (H-14) against darkwinged fungus gnat Bradysia impatiens Johannsen (Diptera: Sciaridae) larvae.
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A 24-h potato-based toxicity test for evaluating Bacillus thuringiensis var. israelensis (H-14) against darkwinged fungus gnat Bradysia impatiens Johannsen (Diptera: Sciaridae) larvae.

机译:基于24小时马铃薯的毒性试验,用于评估苏云金芽孢杆菌变种。以色列(H-14)反对黑翅真菌凤头缓和虫(Diptera:Sciaridae)幼虫。

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摘要

A rapid and inexpensive acute toxicity test was developed and validated to evaluate formulations of the entomopathogenic bacterium, Bacillus thuringiensis Berliner var. israelensis (Serotype H-14) de Barjac (Bti) against laboratory-reared second instar larvae of Bradysia impatiens Johannsen (Diptera: Sciaridae), a pest of greenhouse and nursery crops. The test, based upon larval darkwinged fungus gnat attraction to potato starch as a food source, yielded results in 24 h and was inexpensive to conduct. Using written standard operating procedures, validation data were collected using a Bti formulation from a single lot to validate the method through QA/QC methods and develop a database for employing the toxicity test as a bioassay. The bioassay might be suitable for use with other biological and chemical pesticides, to assist insecticide product development efforts, for quality control testing, for formulation evaluation, and for efficacy comparison between pesticides.
机译:建立了快速而廉价的急性毒性试验,并进行了验证,以评估昆虫致病细菌苏云金芽孢杆菌Berliner var的配方。以色列(H-14型)de Barjac(Bti)对抗温室育苗和育苗作物的害虫Bradysia impatiens Johannsen(Diptera:Sciaridae)实验室饲养的第二龄幼虫。该试验基于幼虫黑翅真菌对马铃薯淀粉作为食物来源的吸引力,在24小时内即可获得结果,并且操作成本低廉。使用书面的标准操作程序,使用Bti配方从单个批次中收集验证数据,以通过QA / QC方法验证该方法,并开发一个将毒性测试用作生物测定方法的数据库。该生物测定法可能适合与其他生物和化学农药一起使用,以协助杀虫剂产品的开发工作,质量控制测试,制剂评估以及农药之间的功效比较。

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