The Assessment of Proliferation Rate of Dental Pulp Stem Cells and Stem Cell from Human Exfoliated Deciduous Teeth by Using Two Different Scaffold

摘要

Introduction: Dental pulp stem cells (DPSC) and stem cells from human exfoliated deciduous teeth (SHED) were identified to be highly proliferative and clonogenic cells.Objectives: The objective was to assess the proliferation rate of DPSC and SHED in presence of two different scaffolds, synthetic granular hydroxyapetite bone graft (Granumas?) and coral scaffolds.Materials and Methods: All teeth were extracted and isolation of the stem cells from dental pulp was done following standard protocol. The stem cells were cultured with a Modified Eagle's Medium (GIBCO/BRL) supplemented with 10% fetal Bovine serum (Equitec-Bio, Kerrville, TX, 100 mum L-glutamine (Wako Pure Chemicals, Osaka), 100 unit/ml penicillin, and 100 mg/ml streptomycin (Biofluids, Rokville, MD). The cells proliferation rate of the DPSC and SHED with scaffolds (Granumas? and coral scaffolds) and without scaffolds was assessed using Alamar Blue (Biosource Internatioal, Inc, USA) and the absorbance of the media was measured spectrophotometrically using ELISA plate reader (Tecan, DKSH) at a wavelength of 570 nm and 600 nm at 0 minute, 60 minutes, 120 minutes, 180 minutes, 240 minutes at day 0, day 4 and day 7.Results and Conclusion: The proliferation rate of DPSC is higher compared to SHED. Cancellous coral scaffold is more suitable in promoting stem cells differentiation and proliferation compared to synthetic hydroxyapetite granular bone graft.