Analysis of Fat-Soluble Vitamins Using UltraPerformance Convergence Chromatography (UPC~2)

摘要

The analysis of fat-soluble vitamins (FSV) formulations, often from oil-filled and powder-filled capsules, or pressed tablets, can be a challenging task. Most often, analysis of these formulations employs a normal phase chromatographic method using traditional normal phase solvents (hexane, tertiary butyl alcohol, ethyl acetate, dichloromethane, and others) that can be expensive to procure and dispose. Other analytical chromatographic techniques for these analyses include reversed phase liquid chromatography, gas chromatography, thin layer chromatography, and colorimetric techniques. The use of UltraPerformance Convergence Chromatography (UPC~2) in fat-soluble vitamin analysis provides a single viable technique that is cost-effective, sustainable, and a green technology alternative that lowers the use of organic solvents, provides fast analysis times, and maintains chromatographic data quality. A series of FSV formulations were analysed using the ACQUITY UPC~2 System. The examined formulations contained vitamin A only, vitamins A + D_3, vitamin E, vitamin D_3 only, vitamin K_1 only, and vitamin K_2 only, as shown in Table 7. Results from these experiments show that UPC~2 has the potential to replace many of the separation methods in use today as the sole technique with no compromises.