Mutagenic Effectiveness and Efficiency of Gamma Rays, EMS and their Synergistic Effects in Blackgram (Vigna mungo (L.) Hepper)

摘要

A comparative study on effect of different dose/concentration of gamma rays, ethyl methane sulfonate (EMS) and combination treatments of gamma rays and EMS on various biological parameters (plant survival, plant height and seed fertility) in Mj generation and frequency of viable mutations induced in the M2generation was carried out in blackgram var. VBN 4 in the present investigation. Mutagenic effectiveness of viable mutants was high in gamma rays treatment than EMS. The efficiency was found to be highest at lower and intermediate concentration of mutagenic treatments. Based on lethality, injury and sterility, gamma rays were more efficient than EMS and combination of both in producing viable mutants. Combination treatments in general proved to bemost effective followed by physical mutagens and EMS in inducing maximum frequency of mutations.