Acclimatization of the Tissue culture Plantlets of Amomumsubulatum Roxb in Different medium Substrates

摘要

The successful acclimatization of the tissue culture plant of large cardamom has been established. The ex-agar well rooted plantlets has been taken for the Ex- Vitro acclimatization. The plantlets has been transplanted in portray filled with differentsubstrate medium which are kept in different conditions in poly tent, relative humidity (50%- 80%), temperature (25 to 35 degree centigrade). Different prophylactic treatment has been given to avoid any occurrence of any fungal attack. Observation of all the parameters has been done for one month.Result showed that. In case of Primary hardening the maximum survival rate of 98% has been noticed in Trail (T4), in standard condition of, (Substrate - coco peat 50% + Rice husk 40% + Perlite 10%), relative humidity under the tent is 80% to 90%, temperature ranging from 25 to 35 degree centigrade, under dull light intensity.In secondary hardening the maximum survival rate of 90% has been noticed in the Trail (T3). (Substrate - Vermicompos, 80%+soil 10%+cocopeat 10% ), relative humidity 50% to60%, temperature ranging from 25 to 40 degree, under 50% shad( net condition.