Slow motions in nondeuterated proteins: Concerted chemical shift modulations of backbone nuclei

摘要

A simple method designed to measure autorelaxation rates of double- and zero-quantum coherences DQC/ZQC{C'N} involving a carbonyl C' and the neighboring amide N nucleus in protein backbones provides valuable insight into slow motions in spite of interference both from the attached amide proton HI and from remote protons such as H-alpha in nondeuterated proteins. The method has been applied to human ubiquitin.