...
首页> 外文期刊>International Journal of Neuroscience >Critical role of s465 in protein kinase C-increased rat glutamate transporter type 3 activity.
【24h】

Critical role of s465 in protein kinase C-increased rat glutamate transporter type 3 activity.

机译:s465在蛋白激酶C增加的大鼠谷氨酸转运蛋白3型活性中的关键作用。

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       

摘要

Glutamate transporters, also called excitatory amino acid transporters (EAATs), uptake extracellular glutamate and regulate neurotransmission. Activation of protein kinase C (PKC) increases the activity of EAAT type 3 (EAAT3), the major neuronal EAAT. We designed this study to determine which amino acid residue(s) in EAAT3 may be involved in this PKC effect. Selective potential PKC phosphorylation sites were mutated. These EAAT3 mutants were expressed in the Xenopus oocytes. Phorbol 12-myristate 13-acetate, a PKC activator, significantly increased wild-type EAAT3 activity. Mutation of serine 465 to alanine or aspartic acid, but not the mutation of threonine 5 to alanine, abolished PKC-increased EAAT3 activity. Our results suggest a critical role of serine 465 in the increased EAAT3 activity by PKC activation.
机译:谷氨酸转运蛋白,也称为兴奋性氨基酸转运蛋白(EAAT),摄取细胞外谷氨酸并调节神经传递。蛋白激酶C(PKC)的激活增加了主要神经元EAAT EAAT 3型(EAAT3)的活性。我们设计了这项研究,以确定EAAT3中的哪些氨基酸残基可能参与了PKC效应。选择性潜在的PKC磷酸化位点发生突变。这些EAAT3突变体在非洲爪蟾卵母细胞中表达。 Phorbol 12-肉豆蔻酸酯13-乙酸酯,一种PKC激活剂,显着提高了野生型EAAT3的活性。丝氨酸465突变为丙氨酸或天冬氨酸,而不是苏氨酸5突变为丙氨酸,消除了PKC增加的EAAT3活性。我们的结果表明,丝氨酸465在PKC激活增加EAAT3活性中起着关键作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号