Magnetic Resonance Imaging of Rat C6 Glioma Model Enhanced by Using Water-Soluble Gadolinium Fullerene

摘要

Water-soluble gadofullerene Gd@Ful with composition Gd~(3+)@C_(82)(OH)_X~(3-)(x ~ 20) was synthesized for theranostic study. Nanosuspensions of Gd@Ful were used formagnetic relaxationmeasurements in vitro and formagnetic resonance imaging of a rat with intracranially implanted C6 glioma. Gd@Ful was shown to reduce proton relaxation times in vitro and provide dual contrast of T_1- and T_2-weighted images in a rat brain tumor model after paramagnetic intravenous delivery. Magnetic relaxation times of water protons under action of Gd@Ful were strongly shortened due to cluster formation and increase of motional correlation times of protons in the vicinity of the fullerene cage. The Gd@Ful administration promoted the improvement of glioma contrast enhancement at T_2-weighted images due to accumulation of paramagnetic substance at the tumor site. The contrast efficiency of Gd@Ful corresponds to the characteristics of negative contrast agent. The Gd@Ful nanosuspension is shown to be a contrast enhancer with high antitumor therapeutic potency. The retention of the Gd@Ful in the tumor resulted in a 75 % increase in survival times of the tumor-bearing animals.