PCR-RFLP polymorphism at prolactin locus in buffaloes

摘要

The milk protein and hormone genes are excellent candidate genes for linkage analysis with QTL. Among several hormones that regulate lactation and reproduction in bovines, prolactin is an important anterior pituitary hormone. Allelic diagnosis can besignificantly simplified using PCR amplification and RFLP analysis. Present study was undertaken to detect polymorphism at exon I of prolactin locus using PCR-RFLP in three buffalo breeds.