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CCAAT/Enhancer-binding protein beta regulates expression of human T1R3 taste receptor gene in the bile duct carcinoma cell line, HuCCT1

机译:CCAAT /增强子结合蛋白β调节胆管癌细胞系HuCCT1中人T1R3味觉受体基因的表达

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摘要

The T1R family (T1R1, T I 1R2 and T1R3 receptors) has a role in the detection of umami and sweet tastes in taste buds. Although T1R3 is also expressed in the intrahepatic bile duct, the expression patterns of T1R1 and T1R2 in this region have not been determined. In addition, the mechanisms of transcriptional regulation of the human T1R3 gene (Taslr3) have not been elucidated. In this study, we determined the expression patterns of T1R2 and TIR3 in human liver and the function of C/EBP beta in Taslr3 promoter activity. Immunohistochemistry showed that T1R2 and T1R3 were expressed in the intrahepatic bile duct. 5'-RACE analysis revealed that the transcriptional start sites of Tas lr3 were located 67. bp and 176 bp upstream of the ATG. Promoter analysis of Taslr3 was performed using the luciferase reporter assay and EMSA in the Taslr3-expressing cell line, HuCCT1 The 226-bp region upstream of the ATG had promoter activity, and C/EBP beta activated the Taslr3 promoter activity in HuCCT1 cells. These results show that T1R2 and TIR3 receptors, in addition to their role in taste perception, may also have a role in intrahepatic cholangiocytes. C/EBP was identified as the transcription factor regulating Taslr3 promoter activity in HuCCT1 cells. (c) 2007 Elsevier B.V. All rights reserved.
机译:T1R家族(T1R1,T 1R2和T1R3受体)在检测味蕾中的鲜味和甜味中起作用。尽管T1R3也在肝内胆管中表达,但是尚未确定该区域中T1R1和T1R2的表达模式。此外,尚未阐明人类T1R3基因(Taslr3)的转录调控机制。在这项研究中,我们确定了T1R2和TIR3在人肝中的表达模式以及C / EBP beta在Taslr3启动子活性中的功能。免疫组织化学显示T1R2和T1R3在肝内胆管中表达。 5'-RACE分析显示Tas1r3的转录起始位点位于ATG的上游67.bp和176bp。在表达Taslr3的细胞系HuCCT1中,使用荧光素酶报告基因分析和EMSA对Taslr3的启动子进行了分析。ATG上游226 bp的区域具有启动子活性,而C / EBP beta激活了HuCCT1细胞中的Taslr3启动子活性。这些结果表明,T1R2和TIR3受体除了在味觉中发挥作用外,还可能在肝内胆管细胞中起作用。 C / EBP被确定为调控HuCCT1细胞中Taslr3启动子活性的转录因子。 (c)2007 Elsevier B.V.保留所有权利。

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