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Differential regulation of Ku gene expression in etiolated mung bean hypocotyls by auxins

机译:植物生长素对黄化绿豆下胚轴中Ku基因表达的差异调节

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摘要

Plant Ku genes were identified very recently in Arabidopsis thaliana, and their roles in repair of double-stranded break DNA and maintenance of telomere integrity were scrutinized. In this study, the cDNAs encoding Ku70 (VrKu70) and Ku80 (VrKu80) were isolated from mung bean (Vigna radiata L.) hypocotyls. Both genes were expressed widely among different tissues of mung bean with the highest levels in hypocotyls and leaves. The VrKu gene expression was stimulated by exogenous auxins in a concentration- and time-dependent manner. The stimulation could be abolished by auxin transport inhibitors, N-(1-naphthyl) phthalamic acid and 2,3,5-triiodobenzoic acid implicating that exogenous auxins triggered the effects following their uptake by the cells. Further analysis using specific inhibitors of auxin signaling showed that the stimulation of VrKu expression by 2,4-dichlorophenoxyacetic acid (2,4-D) was suppressed by intracellular Ca2+ chelators, calmodulin antagonists, and calcium/calmodulin dependent protein kinase inhibitors, suggesting the involvement of calmodulin in the signaling pathway. On the other hand, exogenous indole-3-acetic acid (IAA) and α-naphthalene acetic acid (NAA) stimulated VrKu expression through the mitogen-activated protein kinase/extracellular signal-regulated kinase pathway. Altogether, it is thus proposed that 2,4-D and IAA (or NAA) regulate the expression of VrKu through two distinct pathways.
机译:最近在拟南芥中鉴定了植物Ku基因,并仔细研究了它们在修复双链断裂DNA和维持端粒完整性中的作用。在这项研究中,编码Ku70(VrKu70)和Ku80(VrKu80)的cDNAs是从绿豆(Vigna radiata L.)下胚轴中分离出来的。两种基因均在绿豆的不同组织中广泛表达,其胚轴和叶片中的水平最高。外源生长素以浓度和时间依赖性方式刺激VrKu基因表达。生长素运输抑制剂,N-(1-萘基)邻苯二甲酸和2,3,5-三碘苯甲酸可以消除刺激,暗示外源生长素被细胞摄取后触发了这种作用。使用生长素信号传导的特定抑制剂进行的进一步分析表明,胞内Ca2 +螯合剂,钙调蛋白拮抗剂和钙/钙调蛋白依赖性蛋白激酶抑制剂抑制了2,4-二氯苯氧基乙酸(2,4-D)对VrKu表达的刺激。钙调蛋白参与信号通路。另一方面,外源吲哚-3-乙酸(IAA)和α-萘乙酸(NAA)通过有丝分裂原激活的蛋白激酶/细胞外信号调节激酶途径刺激VrKu表达。因此,总共提出了2,4-D和IAA(或NAA)通过两种不同的途径调节VrKu的表达。

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