Development of a Cholesterol Biosensor by Chronoamperometric Deposition of Polyaniline-Ag Nanocomposites

摘要

Chronoamperometry was employed to prepare polyaniline-p-toluene sulphonic acid-silver (PANI-pTSA-Ag) nanocomposite film on indium tin oxide-coated glass plate to fabricate a cholesterol bioelectrode. The authors observed the high sensitivity of 36.3 μA/ mg/dL with a fast response time of 10 s in the range of 20-400 mg/dL for the bioelectrode. The bioelectrode exhibits uniform activity for 50 days under refrigerated conditions. Attempts have been made to utilize this bioelectrode for estimation of total cholesterol in blood serum samples.