Chemotherapy with hybrid liposomes for acute lymphatic leukemia leading to apoptosis in vivo.

摘要

Hybrid liposomes (HL) composed of 95 mol% l-alpha-dimyristoylphosphatidylcholine (DMPC) and 5 mol% polyoxyethylene (25) dodecyl ether (C(12)(EO)(25)) were prepared by sonication. A clear solutions of HL-25 having hydrodynamic diameter of about 50 nm could be maintained over 3 weeks. Remarkable reduction of tumor volume in model mice of acute lymphatic leukemia (ALL) intravenously treated with HL-25 without drugs after the subcutaneously inoculation of human ALL (MOLT-4) cells was verified in vivo. Induction of apoptosis in tumor of model mice of ALL treated with HL-25 was observed in micrographs on the basis of TUNEL method. Remarkable decrease of the ascites in ALL model mice treated with HL-25 was observed. It is noteworthy that prolonged survival (>400%) was obtained in model mice of ALL after the treatment with HL-25 without drugs.