Plant Regeneration from Leaf Explants of Clematis gouriana Roxb. - An Endemic Medicinal Plant of Western Ghats

摘要

Studies were undertaken to evaluate the regenerative capacity of leaf explants of Clematis gouriana Roxb. The leaf explants were excised from healthy plants and cultured on Murashige and Skoog medium (MS medium) variously supplemented with growth regulators. Regeneration occurred via organogenesis on MS medium supplemented with 3.5 to 5.5 mg l~(-1) 6-furruryl aminopurine (FAP), 0.2 to 0.6 mg l~(-1) indole-3-butyric acid (IBA) and 0.2-0.6 mg l~(-1) thidiazuron (TDZ). The frequency of shoot bud production was the highest (mean of 10.90 +- 0.27 shoots per explants) at 4.5 mg l~(-1), FAP 0.4 mg l~(-1) IBA and 0.4 mg l~(-1) TDZ. Regenerated shoots formed complete plantlets on medium containing 0.1 to 0.5 mg l~(-1) IBA as well as on MS basal medium without growth regulators. Matured plants were established, acclimatized, and thrived in greenhouse conditions and grew normally without any morphological variation. The regeneration protocol developed in this study provides a basis for germplasm conservationand for further investigation of medicinally active constituents of C. gouriana, a medicinal plant of Western Ghats.